One day, Yoichi who grew up while not knowing his parents has received the news of his father's death. His father left behind a testament——which said he is to pick only one out of five 'little sister candidates'?!
"...I don't want to work, I want to laze around together with Onii-chan." (By Serene)
"Let's have a match! If I win, Nii-chan will... make me his little sister!" (By Tomomi)
"Onii-sama will choose me, isn't that right? It's our destiny." (By Sayuri)
"To become a girl, that's why I want to be Nii-san's little sister!" (By Yuuki)
"If Mii-chan becomes Nii-chama's little sister, she will become rich right?" (By Mika)
The little sisters who were presented to him had more than one or two odd quirks, a slapstick comedy between siblings starts here!
We talk culture 
